The effect of Staphylococcus aureus exposure on white-tailed deer trabecular bone stiffness and yield

Abstract

With a growing number of osteomyelitis diagnoses, many of which are linked to Staphylococcus aureus (S. aureus), it is imperative to understand the pathology of S. aureus in relation to bone to provide better diagnostics and patient care. While the cellular mechanisms of S. aureus and osteomyelitis have been studied, little information exists on the biomechanical effects of such infections. The aim of this study was to determine the effect of S. aureus exposure on the stiffness and yield of trabecular bone tissue. S. aureus-ATCC-12600, a confirmed biofilm producer, along with one hundred and three trabecular cubes (5 × 5 × 5 mm) from the proximal tibiae of Odocoileus virginianus (white-tailed deer) were used in this experiment. Bone cubes were disinfected and then swabbed to confirm no residual living microbes or endospore contamination before inoculation with S. aureus (test group) or sterile nutrient broth (control group) for 72 h. All cubes were then tested in compression until yield using an Instron 5942 Single-Column machine. Structural stiffness (N/mm) and yield (MPa) were calculated and compared between the two groups. Our results revealed that acute exposure to S. aureus, within the context of our deer tibia model, does not significantly decrease trabecular bone stiffness or yield. The results of this study may be of value clinically when assessing fracture risks for osteomyelitis or other patients whose cultures test positive for S. aureus.