Abstract
Oleaginous fungus Mucor circinelloides utilizes different carbon sources associated with sustainable production of lipids, using soybean oil as carbon source, the fungus accumulated more intracellular lipids. Nonetheless, the metabolic changes in M. circinelloides upon vegetable oil as main carbon source have not been yet reported. Therefore, this study was conducted to investigate the metabolomics of M. circinelloides WJ11 cultivated in mixed glucose and soybean oil as carbon source and to reveal its effects on lipid metabolism. M. circinelloides strain WJ11 was cultured under optimized conditions in a fermenter and the biomass samples were collected after 24 h for lipid metabolite investigation. The frozen biomass samples were subjected to various chromatographic analyses like liquid chromatography mass spectrometry analysis and high resolution-mass spectrometry for metabolite profiling. A total of 438 differential metabolites were identified, of which 48 were up-regulated and 33 were down-regulated. Among them, lyso-phosphatidic acid and monoglyceride were up-regulated whereas phosphatidylglycerol and lyso-phosphatidylglycerol were down-regulated in the experimental group (soybean oil and glucose as mixed carbon sources) compared with the control group (glucose as single carbon source). Significant changes in metabolite levels correlated to lipid synthesis were identified. This study showed that the addition of soybean oil to the medium favors the triacylglycerol synthesis in M. circinelloides. Our results can also be applied to the investigation of other microorganisms and would contribute to further genetic engineering for higher lipid accumulation in fungi.
Highlights
Oleaginous microorganisms accumulate lipids which may constitute more than 20% of their dry cell biomass having fatty acids composition almost similar to vegetable oil (Azócar et al, 2010)
Intracellular extracts of M. circinelloides WJ11 grown in soybean oil and glucose were subjected to lipid metabolomics profiling
The results showed that the addition of soybean oil to the culture medium did affect the metabolism of M. circinelloides WJ11
Summary
Oleaginous microorganisms accumulate lipids which may constitute more than 20% of their dry cell biomass having fatty acids composition almost similar to vegetable oil (Azócar et al, 2010). They can be cultivated under controlled conditions, utilizes inexpensive substrates and requires a limited space for their cultivation (Li et al, 2008). In oleaginous fungi excess of carbon in the form hydrophilic substrates such as glucose in the culture media plays an important role in de-novo lipid synthesis (Wynn et al, 2001; Arous et al, 2015; Gujjala et al, 2019; Huang et al, 2018). While excess of carbon in the form of hydrophobic substrates such as cooking oils plays important role in ex-novo lipid
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Biochemistry and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
