THE EFFECT OF SOLVENT TOWARDS ANTIOXIDANT ACTIVITY OF Vernonia amygdalina Delile LEAVES

Abstract

Antioxidants guard against reactive oxygen free radicals. Antioxidants neutralize free radicals by adding electrons to them, preventing bodily harm. African leaves (Vernonia amygdalina Delile.) contain an antioxidant. African leaves are the leaves of a thousand diseases because they have pharmacological effects on various diseases. African leaves contain steroids/triterpenoids, glycosides, saponins, tannins, and flavonoids. This study examined Vernonia amygdalina Delile. leaf combination ethanol extract antioxidant properties. The extracts were prepared with the reflux method using methanol, butanol, isopropanol, ethyl acetate, chloroform, and n-hexane. Antioxidant activities were determined with DPPH, ABTS, FRAP, CUPRAC, O-Phenanthroline, Hydroxyl radical scavenging, total phenolics, and total flavonoids method. The data were analyzed using the Principle Component Analysis (PCA) multivariate exploration technique, which was analyzed using the Minitab software. The highest antioxidant activity was found in African leaf reflux extract with methanol solvent by DPPH method (43.8968 ± 0.021), followed by ABTS (73.6223 ± 0.091), FRAP (89.2924 ± 0.087), CUPRAC (13.4745 ± 0.042), O-Phenantroline (99.0977 ± 0.30), and Hydroxyl radical scavenging (33.5703 ± 0.071). The findings show that Vernonia amygdalina Delile. extract exhibit antioxidant activity. According to the findings, Vernonia amygdalina Delile leaf extract with methanol solvent provided the maximum antioxidant activity.