The Effect of Soil Particle Sizes on Bioremediation Efficiency of Petroleum Contaminated Soils

Abstract

ABSTRACT Bioremediation has been proven to be efficient method of cleaning up oil contaminated soils through the application of proper microorganism to contaminated soils. Hence, this research involves the bioremediation of four different types of contaminated soil. The soils were categorized as laterite, sandy soil, clay soil, and loamy soil which are classified technically using American Society for Testing and Materials (ASTM) method as clayey sand, silty sand, clayey sand and poorly graded sand respectively. These soils were contaminated with Bonny light crude oil to the concentration of 33.3 g/l. The soils were inoculated with petroleum remediation microorganisms. Soil samples were taken after 14 and 25 days, and the residual total petroleum hydrocarbon (TPH) was checked using Environmental Protection Agency (EPA) Method 8015. The condition of the soils was kept almost constant during the process by preserving it in an insulated vessel to avoid evaporation of moist or organic gases. The results obtained showed percentage reduction 70%, 69%, 49%, and 26% in hydrocarbon after the period of 25 days in Sand, Laterite, Topsoil and Clay soil respectively. The removal percentage was the highest (70%) for the sandy soil, and the lowest for the clay soil (26%). The result indicate that the particle size distribution of soil affects bioremediation to an extent, since clay with lowest particle size proved more difficult to bio-remediate.