The effect of soil depth on temperature sensitivity of extracellular enzyme activity decreased with elevation: Evidence from mountain grassland belts.

Abstract

Temperature sensitivity of soil extracellular enzyme activity (EEA), indicated by the temperature coefficient Q10, is used to predict the effect of temperature on soil carbon (C), nitrogen (N), and phosphorus (P) cycling. At present, we lack understanding of elevation and soil depth variations in Q10 of EEA. Here, we measured the Q10 of three enzymes participating in C- (β-1,4-glucosidase, BG), N- (leucine aminopeptidase, LAP), and P- (acid phosphatase, AP) cycling along a vertical grassland belt of China. Soils from five depths (0-10, 10-20, 20-40, 40-60, and 60-100cm) were sampled from three elevations (low, <1000m; middle, 1000-2000m; high, 2000-3000m) and incubated at four temperatures (5, 15, 25, 35°C). The average Q10 of soil EEA ranged from 0.97 to 1.11 and the Q10 of LAP was higher than that of BG and AP. Generally, the Q10 of BG and LAP both increased from low to middle elevation and then decreased, while the Q10 of AP was stable. Moreover, the effect of soil depth on Q10 of EEA was weakened from low elevation to high elevation, and the factors driving Q10 of soil EEA changed with elevation. This study improved the understanding of the vertical pattern of Q10 of soil EEA in water-limited ecosystems, and highlighted that elevation could regulate the effect of soil depth on Q10 of EEA.