Abstract
Mono-diacylglycerol (M-DAG) in this research was produced from the esterification reaction of Palm Fatty Acid Distillate (PFAD) and glycerol. Crude M-DAG from esterification had low purity, due to the remaining free fatty acids, glycerol and triglycerides. Thus, purification with saponification to remove free fatty acids, solvent extractionand crystallization to separate M-DAG from triglycerides was needed. This study aimed to determine the best sodium carbonate (NaHCO3) concentration (2.5, 5, 7.5, and 10%) in saponification reaction and to investigate the effect of saccharides (glucose, GOS, and maltodextrin) addition as a seed in M-DAG crystallization. The best sodium carbonate concentration was 10% (w/w) with the yield of 49.33%, 20.47% FFA content, 0.8% ash, pH 7.28, 69.6% emulsion stability, 0.29% total glycerol, and 0.03% free glycerol. Crystallization with glucose produced the yield of 34.21%, 15.69% FFA, 1.02% ash, 11.02% water content, pH 7.1, 87.82% emulsion stability, 0.35% total glycerol and 0.04% free glycerol. However, addition of saccharides had no significant effect on the quality of pure M-DAG.
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