The Effect of Simultaneous Expression of miR-146a-5p and miR-193a-5p on the Expression of MMP-9 Gene in Colorectal Cancer (HT-29 cell line)

Saeed Noorolyai,Ahad Mokhtarzadeh

doi:10.7575/aiac.abcmed.v.7n.2p.7

Abstract

Background and Purpose: Colorectal cancer is the third leading cause of cancer death in the worldwide. microRNAs are a group of non-coding small RNAs that inhibit target mRNA translation. miR-146a-5p and miR-193a-5p are known as tumor-suppressors, which down expressed in many cancers. In this study, the purpose was to restore the expression level of miR-146a-5p and miR-193a-5p up to normal level, and to investigate their synergetic effects on MMP-9 gene expression in HT-29 cells. Materials and Methods: The HT-29 cell line from colorectal cancer was cultured in RPMI-1640 culture media. Then, miR-146a and miR-193a were transfected with Jet-PEI reagent.The expression of miR-146a, miR-193a and MMP-9 gene in transfected HT-29 cells and control cells were evaluated by using the qR-PCR technique. Statistical analyzes were performed using GraphPad Prism 6 statistical software. Results: Our results show that the expression levels of miR-193a and miR-146a were increased after transfection, and in comparison with un-transfected cells, the expression level of MMP-9 was decreased. Conclusion: The results of this study showed that the simultaneous replacement of miR-146a and miR-193a may act as a potent tumor-suppressor and has an important role in cancer cell migration by influencing molecules such as MMP-9. Thus, simultaneous use of these two microRNAs may be suggested as a potential therapeutic goal in the treatment of colorectal cancer.

Highlights

Cancer is a group of diseases whose main characteristic is unregulated cell growth, invasion and metastasis of cancerous cells from the original site to the rest of the body
Our results show that the expression levels of miR-193a and miR-146a were increased after transfection, and in comparison with un-transfected cells, the expression level of Matrix metalloproteinase-9 (MMP-9) was decreased
The results of this study showed that the simultaneous replacement of miR-146a and miR-193a may act as a potent tumor-suppressor and has an important role in cancer cell migration by influencing molecules such as MMP-9

Summary

Introduction

Cancer is a group of diseases whose main characteristic is unregulated cell growth, invasion and metastasis of cancerous cells from the original site to the rest of the body. One of the most commonly extremely and highly prevalent cancers is colorectal cancer [4]. This is among the most common types of cancers that causes high mortality worldwide [2]. Colorectal cancer is the third leading cause of cancer death in the worldwide. Results: Our results show that the expression levels of miR-193a and miR-146a were increased after transfection, and in comparison with un-transfected cells, the expression level of MMP-9 was decreased. Conclusion: The results of this study showed that the simultaneous replacement of miR-146a and miR-193a may act as a potent tumor-suppressor and has an important role in cancer cell migration by influencing molecules such as MMP-9. Simultaneous use of these two microRNAs may be suggested as a potential therapeutic goal in the treatment of colorectal cancer

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Conclusion