The effect of silencing Kruppel like factor 8 by small interference RNA on proliferation and invasion capability of gastric cancer cell line

Abstract

Objective To investigate the effects of small interference RNA(siRNA)targeting Kruppel like factor 8(KLF8)gene(si-KLF8)on proliferation and invasion of gastric cancer cell MKN-28. Methods Synthesized siRNA targeting KLF8 was transfected into MKN-28, at the same time, gastric cancer cells transfected with negative siRNA were used as control. KLF8 m RNA and protein were detected by using real-time quantitative polymerase chain reaction(Real-time PCR) and Western blotting respectively at 24 h.The viability of cells was examined by using methyl thiazol tetrazolium (MTT)assay at 24, 48 and 72 h.The cell invasion were assessed by Transwell assay. Results Si-KLF8 and negative control siRNA(si-CTRL) were transfected into gastric cancer cells successfully.KLF8 m RNA and protein in si-KLF8 group were lower than in negative control group and normal control group.The proliferations of cells were 0. 125±0. 003, 0. 193±0. 005, 0. 223±0. 005, 0. 267±0. 003 and 0. 315±0. 006 at 24, 48 and 72 h, which were significantly lower than those in negative control group and normal control group.Transwell assay showed that the cell numbers migrated to Matrigel in si-KLF8 group(42. 0±3. 6) were significantly lower than in negative control group and normal control group.However, no significant difference was found between negative control group and normal control group. Conclusion si-KLF8 can inhibit proliferation and invasion capability of human gastric cancer cells, which indicate KLF8 plays an important role in the generation and development of gastric cancer. Key words: Gastric neoplasm; Interference RNA; Kruppel like factor 8