Abstract
The influence of the viability of the organisms of various rates of freezing and thawing of suspensions of M. leprae in 10% glycerol and 0.1% bovine serum albumin was investigated, employing Shepard's mouse footpad technique as a measure of viability. Freezing and thawing produced some loss of viability. Rapid freezing was less deleterious than slow freezing, and approximately 75% of the organisms survived rapid cooling to −60°C and overnight storage at this temperature, followed by slow warming. Study of the additional effect of prolonged storage on the viability of M. leprae is planned.
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