THE EFFECT OF SERUM FACTORS FROM PATIENTS WITH/WITHOUT ESSENTIAL HYPERTENSIOIN ON MEMBRANE RECEPTORS FROM RAT CEREBRAL CORTEX VASCULAR AND CARDIAC MUSCLE CELLS

Abstract

Essential Hypertension (EHT)is one of the main risk factors for Alzheimer's disease(AD). The aim of our study was to point out experimental data on the potential role of some serum factors from blood of patients with (EHT) in determining some changes in the ionic flow and neuromediators uptake by the rat cerebral cortex, cardiac and arterial smooth muscle cell receptors with a view to estimate the relevance of these factors in the development of cardiovascular pathology and (AD) and the possible therapeutic management of these agents under study. Our study has been conducted on 34 patients :17 aged 35–86 with EHT (10 men and 7 women) and 17 normotensive subjects (aged 30–84 years old) hospitalized in NIGG Ana Aslan. The radioactive incubation experiments of rat cerebral cortex, heart and aorta have been done according with the published technique. The uptake of 45CaCl2,3H Serotonine,3H Cortisol and 3H Norepinephrine by animal tissues in the presence of serum from (EHT) and normotensive patients has been assayed using a Beta Berthold Scintilator Counter. Our data have pointed out a a significant increase in 45Ca uptake by cerebral cortex incubated with serum from (EHT) patients while in rat heart and aorta there is a decrease in 45Ca uptake. When incubated with serum from (EHT) patients in the presence of 3H Norepinephrine, the uptake is increased in cerebral cortex and heart and decreased in aorta. In samples incubated with serum from hypertensive patients, the uptake of 3H Serotonine was significantly decreased in rat brain cortex, heart and aorta. in comparison with biological samples incubated with serum from normothensive patients. Regarding 3H Cortisol uptake, a significant increase in brain cortex and aorta has been recorded in the presence of serum from hypertensive patients while in rat heart there was a decrease in 3H Cortisol uptake. According to our experimental results serum factors present in the blood of (EHT) patients are responsible for changes in receptor state behaviour concerning the uptake of 45Ca,3H SErotonine,3H Noradrenaline and 3H Cortisol in rat cerebral cortex as well as in heart and aorta which may have a significance in the onset of (AD) in (EHT) patients.