Abstract
Introduction Ovarian torsion is a serious cause of gynecological surgical emergency. Recently, a conservative approach including detorsion and releasing the pedicle to preserve fertility was advocated. However, detorsion worsens the tissue injury leading to ischemia/reperfusion (I/R) and production of reactive oxygen species. Selenium (Se) is an essential trace element and a component of the antioxidant enzymes that protect the cells against the effects of free radicals. Aim This study was designed to investigate the possible protective effect of Se on I/R-induced injury of ovary in a rat model (using histological and biochemical studies). Materials and methods Twenty-six adult female albino rats were divided into three groups: group I (control), group II (the I/R group) including rats exposed to right ovarian ischemia for 3 h and then reperfused for 12 h, and group III (the Se-treated group) including rats exposed to I/R as group II, in addition to 0.2 mg/kg Se injected intraperitoneally 30 min before reperfusion. Ovarian tissues were excised for histological, immunohistochemical, morphometric, and biochemical studies. Statistical analysis was performed. Results In the I/R group, the right ovary showed secondary follicles with desquamated cells into the antral cavity, congested vessels, multiple distorted follicles, massive extravasated red blood cells (RBCs), multiple dark nuclei, and vacuolations in the corpora lutea. The left ovary recruited congested vessels and extravasated RBCs in the corpora lutea. In the Se-treated group, the right ovary revealed some dark nuclei and vacuolations in some atretic follicles besides few extravasated RBCs. A significant increase in the mean area% of caspase-3 immunoreactivity was found in the right ovary in the I/R group compared with the other groups. A significant increase in DNA fragmentation percentage, a significant decrease in reduced glutathione concentration, a significant decrease in catalase activity, and a significant increase in malondialdehyde concentration were found in the I/R group compared with the other groups. Furthermore, administration of Se restored these values to normal levels. Conclusion Se proved to be effective in preventing tissue damage induced by I/R in rat ovaries.
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