Abstract
Tissue damage mediated by the formation of chemically reactive free radicals, often derived from oxygen, has been implicated as the cause of many diseases in man and animals. However, in many instances the evidence for the involvement of free radicals in disease processes is indirect. This evidence includes formation of breakdown products from free radical mediated destruction of essential cell constituents and the protective effect of free radical scavengers and antioxidant enzymes. Electron spin resonance (ESR) spectroscopy has been used for the characterisation of free radicals in both chemical and biological systems, either directly in the case of stable radicals or after reaction of unstable radicals with a “spin trap” to form a more long lived radical which can be detected. In this report we describe the application of both direct ESR spectroscopy and ESR spectroscopy after spin trapping to investigate free radicals in heart from Se- and vitamin E-deficient rats.
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