The effect of seed presoaking with KNO3 on seed germination, proline, protein pattern, ß-Amylase and mineral compositions of two faba bean cultivars treated with NaCl

Abstract

SEEDS of two broad bean cultivars (Vicia faba, cv. Nobaria 3 and cv. Sakha 3) were obtained from Mallawi Agriculture Research Center, El Minia Governorate, Egypt. Different salinization levels (0, 40, 80, 120 and 160mM NaCl) were used and both cultivars were presoaked in either water (Reference control) or potassium nitrate (3mM), then left for germination for five days. Plants transported to the field and further growth for 65 days from sowing. The final germination percentage the two broad bean cultivars of Vicia faba c were decreased by increasing salinity in the rooting medium. Seed presoaking in KNO3 resulted in remarkable increase in final germination percentage especially in cultivar Sakha 3. Both cultivars accumulated proline in both different organs reached 3 folds higher than absolute control, Seed presoaking in KNO3 had a significant increase in proline contents especially in shoots regardless the salinity levels used. Mineral composition the plants of the two broad bean cultivars showed various responses with increasing salinity. Seed presoaking in KNO3 resulted in remarkable decrease in Na and increased K contents in tested plants. One band of β-amylase were detected under different concentrations of NaCl combined with KNO3. The intensity of the bands varied between various treatments. Exposure of Sakha 3 and Nobaria 3 cultivars to different concentrations of NaCl salinity and those presoaked in KNO3 (3mM) were produced marked changes in their protein pattern.