The Effect of Secretome Hypoxia Mesenchymal Stem Cell (Sh-MSC) Therapy on PDGF and IFN-Gama Gene Expression in Male Wistar Rat Excision Models

Abstract

Wounds are damage to normal anatomical structures and functions due to pathological processes that originate internally or externally and affect certain organs. The wound healing process consists of four highly integrated and overlapping hemostasis phases, inflammation, proliferation, and remodeling or network resolution. Therapy using Mesenchymal Stem Cells (MSC) is considered effective in improving skin wound healing because it has the potential for differentiation and immunoregulation and there is no potential for post-treatment rejection. This research aims to prove the influence of secretome hypoxia mesenchymal stem cells (SH-MSC) on PDGF and IF-Gamma gene expression in Wistar rats using the excision wound model. This research is in-vivo experimental research with Post-test Only Control Group Design. The research subjects were 18 male Wistar rats with excision wound models divided into three groups there are placebo gel (negative control), Clobetasol at a dose of 0.25g/kg (positive control), and the P1 group SH-MSC gel at a dose of 400 μl/kg BW, were given treatment for 5 days. On the 6th day, the skin tissue was examined using the RT-PCR method to see the expression of the PDGF and IF-gamma genes. Statistical analysis using One-way ANOVA and Post Hoc LSD test. The average PDGF gene expression in the P1 group was the highest, followed by the positive control. The lowest average expression of the PDGF gene was found in the negative control. The average IFN-γ gene expression in the negative control was the highest, followed by the positive control group. The lowest average expression of the IFN-γ gene was found in the P1 group. The administration of SH-MSCs topical gel 400μl/kg BW increased PDGF expression in male Wistar rats with excision wound models, whereas the administration of gel secretome SH-MSC at a dose of 400μl/kg BW can reduce IFN-γ levels in male Wistar rats with the excision wound model.