The Effect of Seaweed Extract on The Growth of Shoot of Shallot (Allium wakegi Araki) Lembah Palu Variety on in vitro

Abstract

Plant cultivation using sand media and conventional soil methods often have time and environmental constraint. One method that is expected to support the supply of quality shallots seeds is to propagate the seeds through tissue culture techniques. This study aims to know the effect of seaweed concentration on the growth of local palu shallot shoot in vitro. This research was carried out at the Biotechnology Laboratory, Faculty of Agriculture, Tadulako University, Palu. This study conducted using a completely randomized design (CRD) of one factor consisting of five levels, namely, without seaweed extract, seaweed extract the concentration of 5%, seaweed extract concentration of 10%, seaweed extract. The concentration of 15%, and seaweed extract concentration of 20%. Every treatment was quadruplicate; therefore, there were 20 experimental plots. Every unit of the experiment was planted with two explants; thus, the total sample amounted to 40. The results showed, adding 20% seaweed extract, in general, gave better growth in all parameters of the number of leaves and leaf length indicated by the number of leaves (7.50) and leaves size (10.80 cm).