Abstract
In five beef bulls spermatogenic cells labelled with 3H-thymidine were used to estimate the duration of one cycle of the seminiferous epithelium (S.E.) and epididymal passage (E.P.) of spermatozoa after 10 or 20 hr of scrotal insulation designed to increase testicular temperature. From six autoradiographic estimates in three bulls, the mean value for 1 S.E. cycle was 13.4 days (range 12.9 to 13.6 days). Two estimates of E.P. were both 13.5 days. Scrotal insulation altered both semen quality (lowered percentage live and motile spermatozoa, raised percentage abnormal spermatozoa and lowered concentration per ml spermatozoa) and the histological appearance of the seminiferous epithelium. However, the rate of spermatogenesis and epididymal transport was unchanged.
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