The effect of saturated and non-saturated fatty acids on the filtration pressure of platelet-rich plasma. Correlation with platelet aggregation

Abstract

The influence of the salts of saturated and non-saturated fatty acids from C8 to C22 on the aggregation of human platelets is compared within the framework of 2 different techniques: the test of filtration pressure, in correlation with the classical photometric test of aggregation. Thus, with the technique of filtration pressure of platelet-rich plasma, one finds an increase in filtration pressure as a function of the number of carbon molecules in the chain used. The presence of a double bond generally results in a decrease in filtration pressure. In the classical photometric test the fatty acids induce a progressive and irreversible deviation curve, demonstrating both the aggregation of platelets and the probable bonding of the fatty acids with plasmatic proteins. The presence and intensity of platelet aggregation are related to the molecular weight of the chain of fatty acids under consideration and inhibited by the presence of double bonds. From the similarity of the results obtained with the 2 techniques, one might surmise that the filtration pressure test is a new approach to platelet aggregation.