The Effect of Sapodilla Leaf Extract on IL-6 and TGF- β Levels of Skin Tissue (Experimental Study on Male White Rats of Wistar Strain Exposed to UV Light)

Abstract

Excessive exposure to ultraviolet light affects cell and tissue homeostasis which has a damaging effect on DNA characterized by the formation of reactive oxygen species (ROS). Inflammation due to UV rays is the main cause of oxidative stress in the skin. Healing of wounds caused by sunburn through a complex process consisting of inflammatory cell infiltration, cell proliferation, and tissue remodeling phases to restore normal skin integrity and function, rapid healing and epithelialization to prevent infection and minimize functional and aesthetic complications. The researchers aimed to see the effect of administering sapodilla leaf extract cream (Manilkara zapota) on IL-6 and TGF-β levels in Wistar rats with sunburn. Experimental study with a post test only control group design using a total of 30 mice as experimental animals, divided randomly into 4 treatment groups consisting of a control group, a placebo group, a sapodilla leaf cream group with a dose of 25% and a 50% cream group. Mouse skin tissue exposed to UV was analyzed using the ELISA method to determine the levels of IL-6 and TGF-β. There was no significant difference in the average IL-6 levels between treatment groups with the one way anova difference test, a p value of 0.25 (p0.05), there was a significant difference in the average TGF-β levels for each group with a p value of 0.005 (P0.05), Manilkara zapota leaf extract cream had a significant effect on TGF-β levels at a dose of 50%. Administration of Manilkara zapota leaf extract cream has a dose-dependent effect on IL-6 and TGF-β levels in skin tissue of mice exposed to UV.