Abstract
Problem statement: The aim of study was to investigate the effect of Salivary Gland Extract (SGE) of Lucillia sericata maggots on Human Neonatal Fibroblasts (B-HNF-1) proliferation and migration within collagen/hyaluronan membrane. Chronic, non-healing wounds are major health care problem worldwide. Nowadays, Maggot Debridement Therapy (MDT) has attracted much attention due to its successfull application in a treatment of chronic wounds where conventional treatment has failed. Maggot Excretion/Secretion (ES) contains specific proteinases which are positivelly involved in the remodeling of Extracelallar Matrix (ECM) components. Approach: Immunohistochemical methods using primary monoclonal antibody against marker vimentin was employed for the visualization of fibroblasts cultured within collagen hyaluronan membrane. Results: We found that maggot SGE at concentration of 2.4 and 4.8 gL mL-1 significantly induced the migration of fibroblasts into the membrane after 5 and 10 days of cultivation. On the other hand, SGE at concentration of 7.2 gL mL-1 reduced the migration of fibroblasts. Conclusion: The obtained results suggest that medicinal maggots secretion may speed up the wound healing process through the stimulation of fibroblast migration. However, further detailed research is needed to fully elucidate the substance (s) responsible for fibrloblast stimulation and migration.
Highlights
Sterile maggots of greenbottle blowfly Lucillia sericata (L. sericata) are applied to chronic wounds where conventional treatment has failed. This re-emerging treatment known as a Maggot Debridement Therapy (MDT) is used worldwide due to its efficacy, safety and simplicity
They raises wound pH level from acid to dissected from maggots of L. sericata and subsequently alkaline, which inhibits the growth of bacteria, in homogenised in 1 mL of PBS buffer
The fibroblasts were orientated on the Collagen Hyaluronan Membrane (CHM) longitudinaly
Summary
Sterile maggots of greenbottle blowfly Lucillia (phaenicia) sericata (L. sericata) are applied to chronic wounds where conventional treatment has failed. Allantoin, which is a component of larval secretions, provides an optimal environment for the growth of cells needed for wound healing. Proteolytic breakdown of ECM components by indirect activation, most notably fibronectin and induce fibroblast migration, chemotaxis reepitelisation and tissue remodeling (Gupta, 2008).
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