The effect of Rosa damascenaextract activity on angiotensin converting enzyme and renal functionin Wistar rats induced high diet of sodium

Abstract

Background: Hypertension is known as ‘the silent killer’ because it can appear suddenly and cause various complications which eventually results to death. There are several herbal medicines that can use as antihypertension such as Rosa damascene extract. Objective: The purpose of this research was to determine the effect of Rosa damascene extract (RE) to ACE, renal function (urea, creatinine) and renal histopathology. Methods: The subject of research is male Wistar rat with 150-200 gram in weight, divided into five groups, as follow: P0 = control group; P1 = NaCl 8% + RE 500 mg/kgBW; P2 = NaCl 0.35% + RE 500 mg/kgBW; P3 = NaCl 8% and P4 = Nalco 0.35% for 28 days. After 28 days-treatment, rat blood was taken from the heart for analyzing the ACE activity by ELISA method, while uremic, creatinine by spectrophotometer and renal histopatology assessment using hematoxylin eosin stain method. Results: It was found the difference of ACE activity, urea, creatinine serum in Wistar rat which was before administration of sodium diet with p value< 0.05. Rose flower extract (Rosa damascene) has been proved to be able to decrease the activity of ACE, urea, creatinine serum in Wistar rat which was before given Sodiumdiet with value of p < 0.05. Rose flower extract (Rosa damascene) was able to provide a nephron protection effect. Conclusion: Rose flower extract (Rosa damascene) had activity against ACE inhibition, as well as protection of renal function in rats induced by a high sodium diet.