The Effect of Rolling and Crimping on Cell-Seeded Synthetic Scaffolds for the Development of Transcatheter Valves by Tissue Engineering

Magdalena Scheuer,Christoph Schmitz,Bruno Reichart,Eva Bartok,Bassil Akra,Renè Bombien,Cornelia Fano,Trixi Hollweck,Ulrike Haas,Martin Dauner

doi:10.5539/jmsr.v1n3p1

Magdalena Scheuer, Christoph Schmitz + Show 8 more

Open Access

https://doi.org/10.5539/jmsr.v1n3p1

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Abstract

Transcatheter valve replacement using bioprosthetics is a common procedure, however limited due to graft durability. The aim of this study was to verify the mechanical durability of tissue engineered constructs to crimping necessary for transcatheter deployment. Polyurethane patches were seeded with human vascular cells. Mechanical stress of transcatheter methods was simulated by rolling and crimping. Seeded patches were rolled using a patch-rolling device. For crimping, seeded patches were fixed on a polymer stent and crimped by a conventional crimper. Effect of mechanical strain on cellular coating was analyzed by scanning electron microscopy (SEM), immunocytochemistry (ICC) and immunohistochemistry (IHC). SEM analysis of stressed patches showed a confluent cellular coating. ICC and IHC demonstrated the integrity of cellular layers after rolling and crimping. Mechanical stress does not affect the stability of colonized cells on polyurethane patches. This demonstrates the feasibility of applying tissue-engineering methods for the development of seeded transcatheter heart valves.

Highlights

The standard therapy of valvular diseases is heart valve replacement (Bleiziffer et al, 2009)
Effect of mechanical strain on cellular coating was analyzed by scanning electron microscopy (SEM), immunocytochemistry (ICC) and immunohistochemistry (IHC)
Mechanical stress does not affect the stability of colonized cells on polyurethane patches

Summary

Introduction

The standard therapy of valvular diseases is heart valve replacement (Bleiziffer et al, 2009). For high-risk patients, unsuitable for conventional open surgery, transapical valve replacement by biological valves is a treatment being more and more in common use (Bleiziffer et al, 2009; Conradi et al, 2012; ElBardissi, Shekar, Couper, & Cohn, 2011). It enables a minimal-invasive intervention instead of conventional therapies using heart-lung machine and sternotomy (Kempfert et al, 2011). The patient's own vascular cells can be used as the cover of the synthetic material

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