The effect of reverse transformation agents on α-aminoisobutyric acid uptake in transformed and non-transformed cells

Abstract

It has been reported that dibutyryl cyclic AMP plus testololactone, agents which restore normal morphological properties and contact inhibition of movement to transformed cells, stimulate the net uptake of α-aminoisobutyric acid. In those experiments, cells were pre-incubated with the agents for 90 min; using similar conditions we have confirmed this observation and have shown that the effect is accounted for by a 2.8-fold increase in the V MAX of influx with no change in K M. We have also shown that these “reverse transformation agents” cause an increase in the net uptake of α-aminoisobutyric acid in non-transformed cells as well as in transformed cells.