The effect of resveratrol on chemokines CCR5, CXCL10 in rats with acute gouty arthritis

Abstract

[Abstract] Objective To study the effects of resveratrol on chemokines CCR5,CXCL10 of serum and synovial fluid in acute gouty arthritis of rats.Methods According to random number table,36 Wista rats were divided into six groups:i.e.control group,model group,colchicine group,high dose resveratrol group (HR),medium dose resveratrol group (MR),and low dose resveratrol group (LR).The rats were respectively administrated with normal saline,colchicine suspension,high dose resveratrol,medium dose resveratrol,low dose resveratrol for 7 days,once a day.Rats acute gouty arthritis modles were estalished on the fourth day by monosodium urate solution (concentration of 25 mg/ml,0.05 ml) injected into the ankle cavity,while 0.05 ml normal saline was injected into joint cavity of rats in control group.2,9,24,48,72 hours after model estabilishment,blood samples were collected from inner canthus to detect levels of interleukin (IL)-1β,CCR5,CXCL10.72 hours after model estabilishment,synovial fluid were collected from ankel to detect levels of IL-1β,CCR5,CXCL10.The joint swelling index was observed periodically.Results The levels of IL-1 β in the low,medium,and high doses of resveratrol were (9.63 ± 0.71),(7.67 ± 0.48),and (6.66 ± 0.29) pg/ml respectively,being significantly lower than that in the model group (11.85 ± 0.45) pg/ml (P＜0.05) in joint fluid.The levels of CCR5 in the medium and high doses of resveratrol groups were(4.49 ± 0.25) and (4.65 ± 0.25) ng/ml respectively,being significantly lower than that in the model group (5.64 ± 0.28) ng/ml (P＜0.05).The levels of CXCL10 in the medium and high doses of resveratrol groups were significantly lower than that in the model group [(86.79 ± 2.90) and (89.61 ± 5.47) vs (110.13 ± 4.02) pg/ml,P＜ 0.05].The histopathologic results showed that resveratrol might reduce acute gouty arthritis joint edema,improve inflammatory cells infiltrating.Conclusions The levels of IL-1β,CCR5,and CXCL10 were raised in acute gouty arthritis.Resveratrol effectively controls acute gouty arthritis attack,and the curative effect is dose dependent. Key words: Gouty arthritis; Resveratrol; Chemokine