Abstract
This study investigated the anoxic and aerobic phosphate uptake and release reactions and the fraction of denitrifying phosphate-accumulating organisms (DPAOs) under various initial chemical oxygen demand (COD) and residual COD conditions. The results showed that DPAOs and non-DPAOs could release phosphate when high soluble COD was present. Consequently, the phosphate-uptake potential was dynamic and increased when the initial COD increased, the initial polyhydroxyalkanoates (PHA) increased, and the residual COD decreased. Furthermore, the electron acceptor (oxygen of nitrate) has more significant influence on the phosphate uptake/release characteristics, while the residual COD concentrations have little influence on that. The fraction of DPAOs to phosphate-accumulating organisms was 42% when the initial PHA storage was enough by both DPAOs and non-DPAOs. This was closely related to the relative phosphate uptake (47%) in the anoxic zone of the process.
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