Abstract
The incidence of granulosis virus disease among Pieris brassicae larvae feeding on a synthetic diet and modifications of this diet containing less of certain ingredients has been examined. The content of each of the following ingredients was reduced in different batches of the diet: calcium, magnesium, potassium, mixed minor elements, choline chloride, sugar, casein, ascorbic acid, mixed vitamins and the preservatives Aureomycin, and methyl p-hydroxybenzoate. The virus was either fed to the larvae or transmitted to them transovarially. Only when either the sucrose content or the casein content was reduced was there an effect, i.e., an increase, in the incidence of virus deaths. Further work is in hand to obtain an explanation of these results.
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