The effect of redox fluctuation on carbon mineralization in riparian soil: An analysis of the hotspot zone of reactive oxygen species production

Abstract

Riparian zones are important depositional environments at the catchment scale and provide environmental services such as carbon sequestration. This zone is a highly dynamic interface for oxygen and electron exchange, which confers the basis for reactive oxygen species (ROS) production. However, the differences in soil ROS production and their impact on carbon turnover across various redox locations within the riparian zone remain to be fully elucidated. In this study, we investigated the distribution characteristics and generation mechanism of ROS in riparian soil based on soil samples collected in a three-month field monitoring experiment, with additional incubation experiments conducted to examine the effect of hydroxyl radical (•OH) on soil organic carbon (SOC) mineralization. The obtained results demonstrated that the riverine wetland was the hotspot zone for •OH production, with the production flux of 13.05 μmol kg−1 d−1, which was significantly higher than that in floodplain (7.29 μmol kg−1 d−1) and riverbank soils (8.61 μmol kg−1 d−1). Moreover, •OH levels displayed distinct rhythmic fluctuations, with significantly higher concentrations at low water levels compared to those at high water levels, and remained essentially flat over three cycles. The statistic analysis revealed that the ROS production was highly dependent on reduced species and microbial community structure, which function as biogeochemical batteries and electron shuttles under redox fluctuations. Furthermore, the generated •OH involved in the abiotic mineralization of SOC, contributing to 13.1‒21.8 % of total CO2 efflux. Compared to particulate organic carbon (POC), mineral-associated organic carbon (MAOC) fractions of SOC were more susceptible to •OH attacks. The findings provide a novel insight to comprehensively assess the redox process on riparian carbon turnover.