Abstract
Background: Oxidative stress from exercise can contribute to damaging cells, increasing heat shock protein 70 (HSP70) and suppressing the immune system in the body. This research aimed to determine the antioxidant potential of red-fleshed pitaya extract on HSP70 and cortisol expression in rats which were subjected to strenuous exercise. Methods: The subjects of this research were 32 Sprague Dawley male rats, aged 3 months, with an average weight of 200 g. Red-fleshed pitaya extract was obtained from methanol extraction process; a maceration technique was performed and the extract was concentrated using an air-drying method. Rats were randomly divided into four groups. Group 1 were subjected to strenuous exercise and treated with distilled water only; while Groups 2, 3 and 4 were subjected to strenuous exercise and treated with 100 mg/kg body weight, 200 mg/kg body weight and 300 mg/kg body weight of red-fleshed pitaya extract, respectively. Strenuous exercises in rats was performed by intense swimming of 20 min/day, 3 days a week for 3 weeks. HSP70 expression and cortisol were measured with Enzyme-Linked Immune Sorbent Assay (ELISA) method. Results: There was a significant reduction of HSP70 (p=0.000) and cortisol expression (p=0.000) between the groups. Also, there was a significant difference in the average decreasing of HSP70 expression between group 4 and either groups 1 or 2 (p=0.000). However, a significant difference between groups 4 and 3 was not observed (p=0.813). Lastly, a significant difference was found in the average decrease of cortisol expression between groups 4 and 1 (p=0.000), 2 (p=0.000), and 3 (p=0.000) respectively. Conclusion: Red-fleshed pitaya is potential to be utilized as antioxidant to decrease the HSP70 and cortisol expression.
Highlights
Physical activity is an activity which has various influences and significant effects on the body
This research aimed to investigate the antioxidant potential in red-fleshed pitaya extract on heat shock protein 70 (HSP70) and cortisol expression in rats that were subjected to strenuous exercise
HSP70 expression was decreased across all groups (69.57 vs 46.04 vs 31.47 vs 27.65 pg/mL)
Summary
Physical activity is an activity which has various influences and significant effects on the body. Strenuous exercise tends to trigger free radical compound production This impairs the balance of free radicals and antioxidants as a result of oxidative stress[2,3,4]. Research has discovered that oxidative stress from strenuous exercise reduces performance as it damages cells[5], causing pain and muscles fatigues[6], lowering antioxidant levels[7,8], increasing the expression of heat shock protein 70 (HSP70)[9,10] and suppressing the immune system[11]. Oxidative stress from exercise can contribute to damaging cells, increasing heat shock protein 70 (HSP70) and suppressing the immune system in the body. This research aimed to determine the antioxidant potential of red-fleshed pitaya extract on HSP70 and cortisol expression in rats which were subjected to strenuous exercise. A significant difference between groups 4 and 3 was not observed version 2
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