Abstract
This study is concerned with the hydrolysis of sunflower oil in the presence of lipase from the yeast Candida rugosa. The rate of reaction was studied as a function of lipase concentration and as a function of oil:water ratio. The sequential batchwise addition of water in a batch reactor as a means of controlling glycerol concentration in the reaction mixture has been studied. The potential benefits in terms of improved overall reaction kinetics and final conversion for a process are quantified. The effect of reaction products on the downstream separation of the liquid–liquid product mixture is also quantified, and the experimental relationships between coalescence rate, oleic acid concentration and glycerol concentration are presented. A novel technique to estimate the selective accumulation of enzyme at the liquid–liquid interface is described and confirms significant enhancement of enzyme activity in the vicinity of the interface.
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