The effect of putrescine on DNA methylation on cabbage plant under salt stress conditions

Emine Orhan,Esma Yi̇ği̇der,Furkan Uzundumlu,Murat Aydin

doi:10.3906/tar-1911-38

Abstract

Although studies on tolerance to abiotic stress conditions in cabbage species and varieties have been carried out, these studies have generally been conducted in field and greenhouse pot experiments. In such studies, morphological measurements and evaluations have been made. However, there are deficiencies in physiological and molecular detection studies. Therefore, this study focused on detecting any DNA methylation change in the genome of cabbage seedlings when NaCl salt stress in combination with putrescine Put was applied. Different concentrations of NaCl and Put were applied to white head cabbage Brassica oleraceae L. cv Yalova-F1 . Germination rate, root length, shoot length, seedling index and variation coefficient of germination timewere examined. Eight RAPD primers were used in the CRED-RA analysis. Polymorphism ratios were calculated for each concentration and averages were taken. The results showed that MspI enzyme used in the study was cut from the methylation site and the HpaII enzyme was cut when the methylation did not occur. A total seventy-eight methlation sensitive bands were determined based on the treatments. Class IV was the highest methylation type. This was followed by Class I, Class III and Class II, respectively. Total methylation rate % was varied from 59.0% to 71.8%. It was determined that the full methylation rate % was higher than hemi-methylation rate in all treatments. As a result of this study, it was determined that Put applied in both NaCl stress and stress-free conditions caused demethylation.

Highlights

Vegetables are very diverse group and have been used mainly as food for centuries (Zia-Ul-Haq et al, 2014; Galiana-Belaguer et al, 2018; Arin and Arabaci, 2019; Marsic et al, 2019).Cabbage (Brassica oleraceae L. varcapitata sub. var. alba) has been used in human nutrition for a long time due to its rich nutritional value (Günay, 2005)
To understand the potential role of epigenetic changes on cabbage salinity tolerance, we investigated the epigenetic relationships at DNA methylation level in the presence and absence of salinity stress by using random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques
The germination rate was reduced withthe increase of NaCl concentration

Summary

Introduction

Vegetables are very diverse group and have been used mainly as food for centuries (Zia-Ul-Haq et al, 2014; Galiana-Belaguer et al, 2018; Arin and Arabaci, 2019; Marsic et al, 2019).Cabbage (Brassica oleraceae L. varcapitata sub. var. alba) has been used in human nutrition for a long time due to its rich nutritional value (Günay, 2005). Vegetables are very diverse group and have been used mainly as food for centuries (Zia-Ul-Haq et al, 2014; Galiana-Belaguer et al, 2018; Arin and Arabaci, 2019; Marsic et al, 2019). Because of flavonoids and anthocyanin contents, Brassica group shows antioxidant and anticarcinogenic properties (Singh et al, 2006). Salinity, which is the most important factor affecting soil fertility, is an important environmental factor limiting yield in cabbage (Yokoi et al, 2002; Guangyuan and Kun 2007). Most of cabbage varieties are salt resistant, the cabbage leaves that grown in saline soils are dark colored with dry leaf edges (Larcler, 1995). Salinity is an important abiotic stress factor that negatively affects many metabolic events and reduces cropquality and yield (Deveci and Tuğcu, 2017)

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