Abstract
This study was initiated to investigate the effect of pulsed XeCL excimer laser radiation (308 nm) at 25 and 50 pulses per second at radiation levels of 0, 100 mJ, 200 mJ, and 300 mJ on microorganism contamination of in vitro cultures of Heder a canariensis. Explants were cultured on Murashige and Skoog (MS) medium supplemented with 8 mg/BA and 0.2 mg/NAA. The medium was adjusted to pH 5.7. The cultures were maintained for 16-hours photoperiods at 25.5°C. One day following transfer the explants were treated with pulsed excimer laser radiation. The percentage of contamination was reduced by laser radiation treatments. The effect of 5 0 pulses of radiation was found to increase the petiole length, leaf blade width, and leaf number of explants.
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