Abstract
PTEN is inactivated in a subset of lung cancer; therefore, we investigated the involvement of PTEN inactivation in invasiveness of lung cancer cells. AKT at Ser473 was phosphorylated in several lung cancer cell lines with loss of PTEN expression. Therefore, we created a tetracycline inducible expression system of wild-type PTEN (PTEN-WT) as well as catalytically (PTEN-G129R) and lipid phosphatase (PTEN-G129E) inactive PTEN mutants using the PC14, lung adenocarcinoma cell lines, in which endogenous PTEN expression was not detected and AKT at Ser473 was phosphorylated by Western blot analysis. These results suggest that the PTEN gene cannot induce apoptosis alone, but apoptosis can be achieved by the suppression of other pathways. The absence of PTEN expression gives an invasive and metastatic phenotype to cancer cells, and also while staging of cancer cells that allows the transition to a further stage of cancer.
Highlights
Lung cancer is the leading cause of cancer-related deaths. 80% of lung cancers are non-small cell lung (NSCLC) cancers [1]
Western blot analysis was performed in order to understand whether PTEN wt, PTENG129R, and PTENG129E transfected PC 14 cells reaffirmed PTEN expression
The non-small cell lung cancer cell line (NSCLC) we used in our study, PC
Summary
Lung cancer is the leading cause of cancer-related deaths. 80% of lung cancers are non-small cell lung (NSCLC) cancers [1]. 80% of lung cancers are non-small cell lung (NSCLC) cancers [1]. 2, some of stage 3 patients have undergone surgical intervention and some of them have received radiotherapy and chemotherapy [3]. Tumor cells have an aggressive character while escaping from apoptosis. When EGFR receptor stimulated, it activates the PI3K/AKT/Nf-kB pathway with grb-sos adapter protein and the BAD is phosphorylated and the cell escapes from apoptosis. The PTEN tumor suppressor protein dephosphorylation blocks the PI3K/AKT phosphorylation step. PTEN is a tumor suppressor gene located at the 10q23 region of the 10th chromosome and double acting phosphatase as lipid phosphatase and protein phosphatase. PTEN is a tumor suppressor gene that controls the cell cycle, plays a role in the control of proliferation and apoptosis. The two-point mutation in the GAA sequence of the 129th amino acids of the PTEN cDNA significantly affects the PTEN activity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
