Abstract
Objective: The aim of the study is to investigate the influence of cortisol and Heat Shock Protein 70 (HSP70) on the stressed mice to maturationpromoting factor (MPF) expression intrafollicular.Methods: Experimental laboratory with Randomized Post Test Only Control Group Design was carried out on intrafollicular mice. Divided into twogroups, experimental and control groups. The experimental group was given a 95 dB 4 hours/day noisy exposure for 5 days which was analogous topsychological stress in humans and the control group was not given noisy exposure. Furthermore, both groups were examined for cortisol levels toensure stress in mice. Heat shock protein 70 (HSP70) expression was examined as the main regulatory protein for stress response and Maturationpromoting factor (MPF) expression which is a mediator for oocyte maturation.Results: Psychological stress by 95 dB/4 hours/day noisy exposure for 5 days significantly increased serum cortisol levels in experimental group(p=0.000) and HSP70 expression (p=0.000). The effect of Increased cortisol levels and HSP70 expression significantly decrease in MPF expression(p=0.000).Conclusion: The study concluded that psychological stress could be seen by increasing cortisol and HSP70 expression affected to decreasing MPFexpression intrafollicular
Highlights
Oocyte maturation is defined as the starting and confirmation of the first meiotic division, it is start from the vesicular germinal stage to metaphase II stage, followed by cytoplasmic maturation which is crucial for the fertilization and early embryonic development process
Increasing cortisol levels and Heat Shock Protein 70 (HSP70) expression significantly caused a decrease in maturation promoting factor (MPF) expression (p=0.000)
Our result study showed decreasing expression of MPF in the psychological stress group, it is clearly support the data analysis that highly cortisol levels and HSP70 expressions affect to decreasing MPF expression (Beta=0.769; p=0.000)
Summary
Oocyte maturation is defined as the starting and confirmation of the first meiotic division, it is start from the vesicular germinal stage to metaphase II stage, followed by cytoplasmic maturation which is crucial for the fertilization and early embryonic development process. The Nucleus maturation related to meiotic division until the metaphase II stage, whereas cytoplasmic maturation related to preparation of oocyte cytoplasm for embryo fertilization and development [5]. Oocytes need the ability to reinvent the first meiotic division towards MII and the cytoplasmic changes needed for embryo fertilization and development [20]. The protein activity is modulated by specific kinases and phosphatases that control cellular processes such as cell growth, differentiation, cell cycle and meiotic. During the maturation of meiotic oocytes, the activation of MPF and MAPK as protein kinase plays a dominant role. MPF must be activated so that the cell can transit from G2 to M phase [15]
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