Abstract
Male, weanling rats divided into three groups were maintained for 15 days on a semipurified diet containing either 5% casein fed ad libitum (group 1), 20% casein pair-fed to group 1 (group 2), or 20% casein fed ad libitum (group 3). Animals on day 16 were injected i.p. with 3H-AFB 1 (1.90 mg/kg) and were sacrificed six hours later. In both the control and protein deficient animals, binding of AFB 1 to DNA was greater than that for chromatin protein. In the protein deficient animals, there was a consistent decrease (70%) in binding to chromatin, DNA and chromatin protein. The decrease in binding to nuclear macromolecules in protein deficient animals is correlated with carcinogenicity and mixed function oxidase (MFO) enzyme activity, and the relationships between carcinogenicity, MFO activity, and binding are discussed.
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