Abstract
Abstract Prolonged incubation (6 hours) of adipose tissue from fed and 48-hour-fasted rats resulted in an increased rate of synthesis of fatty acids and glyceride glycerol from glucose uniformly labeled with 14C. However, lipogenesis from acetate-1-14C was increased by prolonged incubation in tissues from fasted animals only. These changes in the rate of lipid synthesis were not attributable to alterations in the content of free fatty acid in the tissue during incubation. The effect of prolonged incubation was demonstrable only in the absence of insulin or when very low concentrations were added. This was masked in the presence of a high concentration of insulin. Prolonged incubation increased the rate of glucose entry, as indicated by the more rapid accumulation of the nonmetabolizable sugar 3-O-methylglucose. It is concluded that the increase in utilization of glucose for lipid synthesis following prolonged incubation can be accounted for by the increased rate of glucose entry into the cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
