Abstract
1. 1. The effect of prolactin on osmotic influx of water into isolated gills of the marine threespine stickleback, Gasterosteus aculeatus L., form trachaurus, was studied by an in vitro technique. 2. 2. Prolactin or the solvent (0·6% NaCl) was injected into the gills via the heart and ventral aorta. Immediately after the injection the heart-gill preparation was isolated and incubated in Ringer solution for 30 min before the individual gill arches were isolated and studied in dechlorinated fresh water. 3. 3. Under this condition, prolactin significantly reduced the weight increase of the isolated gills. Thus, prolactin may reduce the osmotic influx of water into the gills of sticklebacks in fresh water.
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