The effect of production conditions on gene expression levels of inulinase of Aspergillus wentii NRLL 1778

Abstract

In this study, the production of inulinase from Aspergillus wentii, the optimum conditions of that production and how those conditions influence gene expression levels of the enzyme were examined. For inulinase of A. wentii, the time of production was determined as 3 days, the temperature of production as 30°C, the starting pH of the production medium as 6.0, and concentration of Jerusalem artichoke added in to production medium as 3%. When the effect of C and N resources added to growth mediums on inulinase activity was investigated, the highest activity was observed in the medium containing 1% maltose. The medium containing 1% (NH4)2HPO4 was determined to be best growth medium. The enzyme was observed to be stable at pH 5.0-6.0 and to maintain its activity at 50°C for 30 minutes. It was found that gene expression was maximum at 2% Jerusalem artichoke concentration, pH 6.0, 35°C on the 1st day of production. The enzyme gene expression levels were higher compared to other studied resources when 1% cellulose was used as the carbon resource and 1% NH4H2PO4 as the nitrogen resource.