Abstract
To investigate the optimum conditions for extracting gelatin from whitecheek shark (Carcharhinus dussumieri (skin, response surface methodology (RSM) has been adopted. In this research, the effect of NaOH concentration (0.01-1 N), HCl concentration (0.01-1 N), and extraction time (3-8 h) on dependent variables, yield and melting point of the skin gelatin have been studied using central composite design. Based on the individual regression models, predict values of multiple response at optimal condition were %23.222 (yield), and 32.224 °C (melting point). The best pretreatment conditions to get to the optimum properties were 0.01 N NaOH, 0.135 N HCl and 3 h extraction time. The results showed that, NaOH and HCl concentrations had significant effects, but extraction time had no significant effect on gelatin extraction yield and its melting point.
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