The effect of probiotics on ıntestinal motility in an experimental short bowel model.

Mehmet Metin,Ahmet Altun,Gökhan Köylüoğlu

doi:10.1590/s0102-865020200080000004

Mehmet Metin, Ahmet Altun + Show 1 more

Open Access

https://doi.org/10.1590/s0102-865020200080000004

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Abstract

PurposeTo investigate the effect of probiotics on spontaneous contractions of smooth muscle isolated from jejunum and ileum of rat model.MethodsFour rat groups were created (n=8, in each) including control (Group 1), control+probiotic (Group 2), short bowel (Group 3), and short bowel+probiotic (Group 4). Groups 1 and 2 underwent sham operation, Groups 3 and 4 underwent massive bowel resection. Bifidobacterium Lactis was administered in Groups 2 and 4 daily (P.O.) for three weeks. On postoperative week 3, rats were sacrificed, and jejunum and ileum smooth muscle were isolated for organ bath. Muscle contraction changes were analyzed before and after addition of antagonists.ResultsShort bowel group exhibited increased amplitude and frequency of spontaneous contractions. The addition of probiotics significantly decreased enhanced amplitude and frequency of bowel contraction in short bowel group and returned to control values. L-NNA increased amplitude and frequency of contractions in all groups. While indomethacin and nimesulide increased the amplitude in all groups, the frequency was only increased in jejunum. Hexamethonium and tetrodotoxin did not change the contraction characteristics in all groups.ConclusionWe suggest that early use of probiotics may significantly regulate bowel motility, and accordingly improve absorption of nutrients in short bowel syndrome.

Highlights

In recent years, numerous publications have shown that probiotic bacteria are highly beneficial for human health[1,2,3,4,5,6]
The results of our study, which investigated the changes in bowel motility and the effects of probiotic treatment on an experimental short bowel model we created in rats, are presented below
No statistically significant difference was detected between the contraction responses of the ileal and jejunal smooth muscles of the control, short bowel, control+probiotic, and short bowel+probiotic groups in 80 mM KCl (P = 0.095, P = 0.467, respectively) (Table 1) (Fig. 1)

Summary

Introduction

Numerous publications have shown that probiotic bacteria are highly beneficial for human health[1,2,3,4,5,6]. A lot of clinical research has been conducted to understand the underlying physiopathology of SBS, the number of studies that examine the effects of the disease on the bowels in vitro is little if any[29] With this goal, we created an animal model of short bowel syndrome in rats and examined the functional changes that occurred over a three-week period. We created an animal model of short bowel syndrome in rats and examined the functional changes that occurred over a three-week period We formed another group that was fed with probiotics that are claimed to have beneficial clinical effects, and we evaluated the changes caused by the probiotics. We tried to explain the reasons underlying the changes in the presence of antagonists which aimed to target different pathways participating in the smooth muscle contraction

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Results

Discussion

Conclusion