The effect of probiotic and prebiotic-MOS on the survival, growth and immune competence on spiny lobster Panulirus homarus culture

Abstract

The use of probiotics and prebiotic to improve growth and health status of spiny lobster has been evaluated. The purpose of this study was to obtain the effect of probiotics and prebiotics for the culture of spiny lobster Panulirus homarus. The method was initiated with culturing of probiotics (4 strains) and supplemented it in a moist diets. Spiny lobster was collected from Jembrana-Bali waters with an initial body weight of 70.34 ± 4.5 g and cultured in concrete tanks with volume of 4 m3. Initial stock density for each treatment was 15 pcs / m3. The treatments tested were supplemented on moist diets with (A) probiotics, (B) probiotics and prebiotics-MOS (Mannan Oligo Sacharida) and (C) Controle (without probiotics and prebiotic) and each treatment with three replication. The results obtained that the survival rate of spiny lobster was not significantly different (P> 0.05), i.e. (A) 92.70%, (B) 93.33 (%) and (C) 93.33% respectively. However, the results of probiotics supplementation as well as a combination of probiotics and prebiotics showed growth differences compared to control (P<0.05), namely (A) 156.97 ± 6.17 g, (B) 153.75 ± 9.17 g, while (C) control 131.47 ± 7.91 g. The probiotics supplementation on moist diets could increased health status of spiny lobsters, this was expressed by target genes related to immunity (ALFHa-1, ALFHa-4, SAA, ProPO, Tgase and CP). Spiny lobster immunity increased by 2.60 to 42.7 times after challenging with MHD (Milky Haemolymph Disease). The supplementation of probiotics and prebiotics (MOS) could increase immune response by 2.10 to 25.75 times, respectively after challenging with MHD.