The effect of prebiotic pretreatment on histological lesions of Nile tilapia fry (Oreochromis niloticus) exposed to silver nanoparticles

Abstract

The presence of emerging nanoparticle contaminants in aquatic environments reduces the safety performance of aquatic animals, so the use of safety stimuli is essential. The aim of the present study was to investigate the effect of different prebiotic levels of oyster mushrooms (Pleurotus ostreatus) on tissue damage in the liver and gills of tilapia exposed to silver nanoparticles. For this purpose, 120 juveniles of Tilapia (Oreochromis niloticus) In 4 experimental groups with 3 replications including treatment 1 as a control (fed with food without probiotics), treatment 2, fed with food containing 0.05% probiotics, treatment 3, fed Were fed with food containing 0.1% prebiotics and treatment 4, fed with food containing 0.2% prebiotics and cultured for 42 days, then silver nanoparticles with a concentration of p5 ppm for 16 days. 0. was added to the culture medium of each of the treatments. At the end of the course, the fish were anesthetized with clove anesthetic solution (220 mg / L) and their liver and gill tissues were isolated for histological studies. Treatments exposed to silver nanoparticles showed extensive complications in gill tissue as well as severe injuries and bile stasis in liver tissue. However, the use of oyster mushroom prebiotics was able to reduce the destructive effects of silver nanoparticles on these tissue complications. Treatment of 0.2% with oyster mushroom prebiotics in the diet can have the best protective effect on tissue damage in the liver and gills of tilapia exposed to silver nanoparticles with a concentration of 0.5 ppm.