The Effect of Polyporus umbellatus(Chinese medicine)on LPS-induced Viability and Cytokine Expression in RAW264.7 cells

Abstract

Event Abstract Back to Event The Effect of Polyporus umbellatus(Chinese medicine)on LPS-induced Viability and Cytokine Expression in RAW264.7 cells Xing Zeng1* 1 Second Affiliated Hospital, Guangzhou University of Chinese Medicine, China Polyporus umbellatus is a medicinal fungus with mild taste and neutral property. The investigations of immunological action of Polyporus umbellatus focus much more on ingredients than the compound of Polyporus umbellatus. The mechanism researches on toxicity attenuation and efficacy potentiation effects of Polyporus umbellatus in recent years reveal that the interaction among the various ingredients from Polyporus umbellatus probably account for the bidirectional regulation of Polyporus umbellatus prescription on immunity. To investigate the immune regulatory function of Polyporus umbellatus by studying its impact on LPS-induced expression of cytokines (IL-1β, IL-6 and IL-10) in RAW264.7 cells, based on our previous studies. RESULTS: In comparison to LPS group, LPS-induced expression of IL-1β, IL-6 and IL-10 in RAW264.7 cells was inhibited by Polyporus umbellatus in a dose-response manner. IL-10 mRNA was promoted by 0.05μg/mL Polyporus umbellatus while inhibited by 0.5 and 5μg/mL.Polyporus umbellatus is capable of attenuating stimulation of LPS on RAW264.7 cells by suppressing the expression of cytokines like IL-1β and IL-6 as well as elevating the antiinflammatory cytokine IL-10. The correlation between the dose of Polyporus umbellatus and IL-10 expression indicates the bidirectional regulatory effect of Polyporus umbellatus on LPS-induced expression of cytokines in RAW264.7 cells.