Abstract
The use of nanomedicines for cancer treatment has been widespread. Fullerenes have significant effects in the treatment of solid tumors. Here, we are going to study the effects of hydroxylated fullerene C60(OH)n(n = 18–22) treatment on chronic myeloid leukemia cell proliferation and investigate its toxicity. The results showed that hydroxylated fullerene C60(OH)n (n = 18–22) at low concentrations (less than 120 μM) not only had apparent toxic side effects, but also promoted the growth of K562 cells, while a high concentration of C60(OH)n had different degrees of inhibition on K562 cells. When the concentration is higher than 160 μM, the K562 cells showed morphological changes, the mitochondrial membrane potential decreased, the cell cycle was blocked in the stage of G2-phase, and cell apoptosis occurred, which may cause apoptosis, autophagy, and a variety of other damage leading to cell death. Meanwhile, it also indicated that its inhibition of solid tumors might be related to the tumor microenvironment; we verified the safety of fullerene without apparent cellular toxicity at a specific concentration.
Highlights
Since its discovery in 1985, fullerene has received much interest and been under investigation as a carbon nanomaterial [1]
These obtained results clarify that fullerene derivative nanomaterials are potential candidates for cancer drugs with high efficacy and low toxicity [8,17], which may open a new perspective for biomedicine. [5] demonstrated the superiority of C70-Lys compared with C70-Ala against the chemotherapy injuries that induced by doxorubicin
The cells were cultured in a medium containing C60(OH)n for 24 h, and there was no obvious regular change in cell proliferation, while the cells were cultured in the same medium for 48 h and the inhibitory effect of C60(OH)n on proliferation began to appear and was relatively stable (Figure 2)
Summary
Since its discovery in 1985, fullerene has received much interest and been under investigation as a carbon nanomaterial [1]. Using the luciferaseexpressing mouse breast cancer cell line 4T1-luc as a xenograft model, Chunying Shu et al [16] discovered that activated Gd-Ala could make full use of oxygen in blood vessels to create ROS, resulting in partial or complete vascular disruption These obtained results clarify that fullerene derivative nanomaterials are potential candidates for cancer drugs with high efficacy and low toxicity [8,17], which may open a new perspective for biomedicine. Finding leukemia treatment medications with high efficacy and minimal toxicity is a pressing issue [28] Throughout these studies on the effect of fullerenes on cancer—most of them focused on the effect of fullerenes in solid tumors [16,19]—there is little research on the specific non-solid tumor cancer: blood cancer. Even though K562 can be considered as a model system for myelogenous leukemia, it is a transformed/immortalized cell line; all experimental results are focused on this cell line
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
