Abstract
Biotechnological approaches provide an efficient and expeditious system for developing agronomic features of rice at the cellular and molecular level compared to traditional breeding methods. The in-vitro regeneration method optimization of recalcitrant indica rice genotypes is a critical step for the biotechnological approaches for further development as the optimum concentrations of Plant Growth Regulators (PGRs) for in-vitro regeneration vary from genotype to genotype. Here, 12 important rice genotypes, including local cultivars and varieties, were collected from different regions of Bangladesh and evaluated for their in-vitro regeneration potentiality. Mature dehusked sterilized rice seeds were inoculated on different basal media (MS, N6, B5) supplemented with different concentrations of 2,4-Dichlorophenoxyaceitic acid (2,4-D) for callus induction. Induced calli were transferred to N6 media supplemented with different combinations of 6-Benzylaminopurine (BA) and 1-Napthaleneacetic Acid (NAA) for shoot induction. Regenerated shoots were rooted on half-strength MS media without supplementation of any hormones. Treatments of different concentrations and combinations of plant growth regulators showed varying results. Maximum callus induction frequencies were observed with 2.5-3.0 mg/l supplementation of 2,4-D on N6 media where Saat, Jamaibabu and Nimikhanai showed the highest callus induction frequencies (84.44%; 78.89 and 80% respectively) and maximum shoot initiation was observed at 2.5 mg/l BA +1.0 mg/l NAA and 3.0 mg/l 6-BA +1.5 mg/l NAA supplemented media where rice genotypes BRRI Dhan71, Nayapaijam, Nimikhanai and BRRI Dhan28 showed the highest shoot induction frequencies-86.67, 85.00, 86.67 and 88.33% respectively. Nearly all of the induced shoots showed root formation on half-strength MS media. This study demonstrates variations in regeneration response of the rice genotypes where cultivars Jamaibabu, Nayapaijam and Nimikhanai showed the highest in-vitro regeneration potential.
Highlights
Rice is a semi-aquatic grass belonging to the Poaceae family, grown mostly in tropical and sub-tropical regions and more than half of the world’s population take rice as a staple food (Gnanamanickam, 2009)
The partially salt-tolerant rice genotypes were collected from Satkira, Khulna and Bagerhat regions of Bangladesh.The partial submergence tolerant local rice genotypes were collected from haor areas of Bangladesh, and the BRRI Dhan28 and BRRI Dhan29 were collected from Bangladesh Rice Research Institute (BRRI)
We evaluated the impact of light on callus induction
Summary
Rice is a semi-aquatic grass belonging to the Poaceae family, grown mostly in tropical and sub-tropical regions and more than half of the world’s population take rice as a staple food (Gnanamanickam, 2009). Out of 23 species, only two species (Oryza sativa and Oryzaglaberrima) are well known for their commercial value, while the two subspecies Oryza sativaindica and Oryza sativa japonica, are widely cultivated in Asia (Linares, 2002). Bangladesh is a major rice-producing country holding the fourth position in rice production throughout the world (Wright, 2020). Rice production in Bangladesh is increasing day by day, and Bangladesh is nearly self-dependent in rice production. Aus and Boro are three major rice groups cultivated in three overlapping seasons, and each rice group has numerous rice germplasms with distinct agro-ecological and climatic niches.
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