The effect of plant-associative bacteria ( Azospirillum and Pantoea) on the fruit quality of sweet pepper under limited nitrogen supply

Abstract

This study investigates the influence of a commercial product, Biopron ®, consisting of the bacteria Azospirillum brasilense and Pantoea dispersa on sweet pepper fruits ( Capsicum annuum L.) under limited N supply. When the N supply was reduced from 12 (control) to 7 mmol L −1, the concentration of total-N in the fruits was significantly reduced in both inoculated and non-inoculated plants. The N supply or inoculation did not affect the dry matter content or fruit firmness, but non-inoculated fruit with low N showed a decrease in pericarp thickness and a significant increase in the color parameter a * compared with the control. Under limited N, inoculation increased the concentration of citric, ascorbic and succinic acids in green fruit compared with non-inoculated fruit, which showed lower values than control fruit. At a later (yellow) stage of development, only succinic acid showed a response to inoculation. Fruit peroxidase (EC 1.11.1.7) activity in fruit of inoculated plants was lower than that observed for non-inoculated fruit grown at both high- and low-N. In contrast, in yellow fruit, total phenolic compounds were increased under N limitation, with no inoculation effect. Our study shows that the effect of plant associative bacteria is not directly related with the increased potential availability of nutrients for uptake, especially for fruit quality characteristics.