THE EFFECT OF PHYTOHORMONES AND LIGHT ON THE GERMINATION OF APPLE POLLEN WITH REDUCED VIABILITY

A V Pavlov,V G Verzhuk,D D Bondaruk

doi:10.30901/2227-8834-2019-4-27-31

A V Pavlov, V G Verzhuk + Show 1 more

Open Access

https://doi.org/10.30901/2227-8834-2019-4-27-31

Copy DOI

Abstract

Background. Studying the effect of phytohormones and light on the germination of apple pollen with reduced viability on an artificial nutrient medium is of importance, because it may facilitate an increase in the germination percentage among plant samples promising for breeding after their long-term preservation under low temperatures.Materials and methods. Pollen viability of the apple-tree cultivar ‘Krasnolistnaya’ was measured by germinating it on an artificial nutrient medium containing 10% sucrose solution and 0.8% agar. Drops of a distilled water suspension of pollen with added phytohormones were applied to the surface of the nutrient medium. Pollen was germinated in the dark (24 hours in a thermostat at 21°C) and under light (in an artificial light chamber at 21°C with a photoperiod of 16 hours of light / 8 hours of darkness). Results. Pollen of cv. ‘Krasnolistnaya’ with reduced viability most effectively germinated with the use of gibberellin at a concentration of 1 mg/l and 10 mg/l: the germination percentage was 22.3±0.8% and 21.8±1.3% respectively (сf. 10.9±1.5% in the reference). The most effective combination of phytohormones was gibberellin 10 mg/l + kinetin 10 mg/l – the percentage of germination was 22.8±6.3%; kinetin 1 mg/l + indolylbutyric acid 1 mg/l – the percentage of germination was 17.5±5.9% vs. 10.9±1.5% in the reference.Conclusion. Phytohormones were observed to have a significant effect on the germination of apple pollen with reduced viability. Effective concentrations and combinations of phytohormones that promote the germination of pollen with reduced viability have been identified. Light does not affect the germination of pollen with reduced viability.

Highlights

Studying the effect of phytohormones and light on the germination of apple pollen with reduced viability on an arti icial nutrient medium is of importance, because it may facilitate an increase in the germination percentage among plant samples promising for breeding after their long-term preservation under low temperatures
Drops of a distilled water suspension of pollen with added phytohormones were applied to the surface of the nutrient medium
‘Krasnolistnaya’ with reduced viability most effectively germinated with the use of gibberellin at a concentration of 1 mg/l and 10 mg/l: the germination percentage was 22.3±0.8% and 21.8±1.3% respectively

Summary

Background

Studying the effect of phytohormones and light on the germination of apple pollen with reduced viability on an arti icial nutrient medium is of importance, because it may facilitate an increase in the germination percentage among plant samples promising for breeding after their long-term preservation under low temperatures. Phytohormones were observed to have a signi icant effect on the germination of apple pollen with reduced viability. Effective concentrations and combinations of phytohormones that promote the germination of pollen with reduced viability have been identi ied. Оптимальнои концентрациеи сахарозы в среде для проращивания пыльцы яблони, по нашим данным, является 10% (Verzhuk et al, 2017). Поскольку ранее (Pavlov et al, 2018) было показано положительное влияние фитогормонов и освещенности на прорастание качественнои пыльцы яблони, хранившеися при –18°С, для решения поставленнои задачи применили обработку состареннои пыльцы с пониженнои жизнеспособностью регуляторами роста растении (фитогормонами), принадлежащими к трем основным группам (кинетины, гиббереллины, ауксины), в различных сочетаниях и по отдельности, одновременно с проращиванием пыльцы на свету и в темноте

Материалы и методы исследований

Findings

Результаты и обсуждение