The effect of photosynthetically active radiation and temperature on the photosynthesis of two Vietnamese species of Sargassum , S. mcclurei and S. oligocystum , based on the field and laboratory measurements

Abstract

SUMMARY The effects of photosynthetically active radiation (PAR) and temperature on the photosynthesis of two Vietnamese brown algae, Sargassum mcclurei and S. oligocystum (Fucales), were determined by field and laboratory measurements. Dissolved oxygen sensors and pulse-amplitude modulated (PAM) fluorometry were used for the measurements of photosynthetic efficiency. A Diving-PAM revealed that underwater measurements of the effective quantum yield (Φ PSII ) of both species declined with increasing incident PAR, with minimum Φ PSII occurring during noon to early afternoon. Φ PSII recovered in the evening, indicating photo-adaptation to excessive PAR. In laboratory experiments, Φ PSII also decreased under continuous exposure to 1000 μmol photons m−2 s−1; and full recovery occurred after 12 h of dark acclimatization. The net photosynthesis – PAR experiments of S. mcclurei and S. oligocystum conducted at 28°C revealed that the net photosynthetic rate quickly increased at PAR below the saturation irradiance of 361 and 301 μmol photons m−2 s−1 and nearly saturated to maximum net photosynthetic rates of 385 and 292 μg O2 gww − 1 min−1 without photoinhibition, respectively. Gross photosynthesis and dark respiration experiments determined over a range of temperatures (12–40°C), revealed that the maximum gross photosynthetic rates of 201 and 147 μg O2 gww − 1 min−1 occurred at 32.9 and 30.7°C for S. mcclurei and S. oligocystum, respectively. The dark respiration rates increased exponentially over the temperature ranges examined. The estimated maximum value of the maximum quantum yield occurred at 19.3 and 20.0°C and was 0.76 and 0.74, respectively. Similar to the natural habitat of the study site, these two species tolerated the relatively high temperatures and broad range of PAR. The ability of these species to recover from exposure to high PAR is one of the mechanisms that allow them to flourish in the shallow water environment.