The Effect of Photoperiod on Adrenocortical Stress Response in Mountain Chickadees (Poecile gambeli)

Abstract

Birds respond to environmental changes by modulating their levels of plasma corticosterone, a stress hormone produced by the adrenal glands. Baseline levels of corticosterone and the magnitude of adrenocortical response to acute stress are known to vary seasonally. Photoperiod is one of several potential factors which could affect the seasonal modulation of corticosterone secretion. In this study, we examined the effects of photoperiod on baseline and acute-stress-induced levels of corticosterone in adult mountain chickadees (Poecile gambeli). We exposed 20 mountain chickadees to short days (8L:16D h) for 60 days, after which half of the chickadees were switched to long days (14L:10D h). We collected blood samples from both groups 33 days after the switching. Our results showed that photoperiod had no significant effect either on baseline levels of corticosterone or on the maximum levels reached during the adrenocortical response to acute stress. However, photoperiod had a significant effect on the way that chickadees responded to acute stress: birds maintained on long days reached significantly higher levels of corticosterone between 5 and 20 min after capture than birds maintained on short days, which reached their maximum levels of corticosterone between 20 and 50 min. Females reached significantly higher levels of corticosterone than males in response to acute stress. Our results suggest that factors other than photoperiod are responsible for the observed seasonal changes in baseline levels of corticosterone, whereas photoperiod is directly involved in regulation of adrenocortical stress response.