The effect of phosphate concentration on phytase production and the reduction of phytic acid content in canola meal by Aspergillus carbonarius during a solid-state fermentation process

Abstract

The use of canola meal, an abundant side-product of canola oil processing in Canada, as animal feed is hampered by high phytic acid levels that reduce metal cation availability. Aspergillus carbonarius grows well in a solid canola meal medium, produces phytase and reduces the phytic acid content to zero. Inorganic phosphate addition at a concentration of 1 mg and 5 mg/110 g solid-state culture system results in better growth of the microorganism, higher rates and levels of phytase production, and faster reduction of phytic acid content. Phosphate concentrations of 50 mg and 100 mg/110 g inoculated system had a negative effect affecting primarily the initial rates of biomass and phytase production and phytic acid content reduction. Models that predict biomass production (expressed as glucosamine content) and phytase, as well as the reduction of phytic acid content in the solid-state cultures supplemented with phosphate are reported. They fit the experimental results reasonably well (with a maximum deviation of 7%).