The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles

Ekaterina Bobrikova,Alexey Chubarov,Elena Dmitrienko

doi:10.3390/magnetochemistry7090128

Abstract

Magnetic Fe3O4 nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, such as pH, buffer components, and oligonucleotides sequences, were chosen for binding efficiency studies and oligonucleotide binding constant calculation. Standard characterization techniques, such as dynamic light scattering, zeta potential, and transmission electron microscopy, provide MNPs coating and stability. The toxicity experiments were performed using lung adenocarcinoma A549 cell line and high reactive oxygen species formation with methylene blue assay. Fe3O4 MNPs complexes with oligonucleotides show high stability and excellent biocompatibility.

Highlights

Metal nanoparticles represent a significant doorway for various biomedical applications [1,2,3,4,5,6,7]
Magnetic mixed iron oxide nanoparticles were by synthesized by the coprecipitation
The technique consists of short incubation Magnetic nanoparticles (MNPs) with oligonucleotides in optimal buffer conditions

Summary

Introduction

Metal nanoparticles represent a significant doorway for various biomedical applications [1,2,3,4,5,6,7]. The use of nanoparticles as carriers for therapeutic molecules has been investigated to improve the therapeutic effect and avoid side effects. One of the approaches is to incorporate a magnetic core into the nanoparticle structure. Magnetic nanoparticles (MNPs) have become a vital nanomedicine material for drug delivery, biosensing, and biocatalysis [1,2,3,4,8,9,10,11,12]. MNPs can be directed around the body with a magnetic field, allowing for directed treatment, and drug/gene delivery strategy. MNPs provide therapy and diagnostic construction developments which are called theranostics

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