Abstract
Magnetic Fe3O4 nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, such as pH, buffer components, and oligonucleotides sequences, were chosen for binding efficiency studies and oligonucleotide binding constant calculation. Standard characterization techniques, such as dynamic light scattering, zeta potential, and transmission electron microscopy, provide MNPs coating and stability. The toxicity experiments were performed using lung adenocarcinoma A549 cell line and high reactive oxygen species formation with methylene blue assay. Fe3O4 MNPs complexes with oligonucleotides show high stability and excellent biocompatibility.
Highlights
Metal nanoparticles represent a significant doorway for various biomedical applications [1,2,3,4,5,6,7]
Magnetic mixed iron oxide nanoparticles were by synthesized by the coprecipitation
The technique consists of short incubation Magnetic nanoparticles (MNPs) with oligonucleotides in optimal buffer conditions
Summary
Metal nanoparticles represent a significant doorway for various biomedical applications [1,2,3,4,5,6,7]. The use of nanoparticles as carriers for therapeutic molecules has been investigated to improve the therapeutic effect and avoid side effects. One of the approaches is to incorporate a magnetic core into the nanoparticle structure. Magnetic nanoparticles (MNPs) have become a vital nanomedicine material for drug delivery, biosensing, and biocatalysis [1,2,3,4,8,9,10,11,12]. MNPs can be directed around the body with a magnetic field, allowing for directed treatment, and drug/gene delivery strategy. MNPs provide therapy and diagnostic construction developments which are called theranostics
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
