The Effect of Petai (Parkia Speciosa Hassk) Seed Extract and Glimepiride on Blood Sugar Levels Of Alloxan-Induced Mice (Mus Musculus)

Abstract

Diabetes mellitus is a metabolic disease characterized by hyperglycemia as a result of insulin deficiency in the body, ineffective insulin function, or both. Glimepiride is a third generation of oral hypoglycemic drug (OHO) that has a lower risk of hypoglycemia and weight gain compared to the first generation of sulfonylurea. Petai plant (Parkia speciosa Hassk) is one of the medicinal plants that has been known to be efficacious as antioxidant and anti-diabetic. This study aimed to discover the effect of administering the seeds of petai (Parkia speciosa Hassk) on blood glucose levels in mice induced by alloxan. This experimental study used 42 mice divided into 5 groups, they were K- (a standard diet), K+ (alloxan 175 mg/kgBB), control treatments P1 and P2 with doses of 300 mg/kgBB and 400 mg/kgBB of petai seed extract, and control treatment P3 with Glimepiride 0.0026/20g. This research was conducted at Pharmacology Laboratory of the Faculty of Pharmacy and Biochemistry of the Faculty of Medicine, Andalas University. Blood sugar levels were measured by the colorimetric method using vitalab micro spectrophotometer (Microlab 300). The results of the Kruskal-Wallis test on the blood sugar levels of mice with the administration of petai seed extract at a dose of 300mg/KgBB with a mean decrease in blood sugar levels of 85.48±5.32 mg/dl and on the blood sugar levels of mice with the administration of Glimepiride 0.0026mg/20gBB with a mean decrease in blood sugar levels of 88.73±9.95 mg/dl for 7 days did not show a statistically significant difference in the Post Hoc Test with Mann-Whitney (p = 0.423) with a mean difference of reduction of 3.25mg/dl. The conclusion of this study is that the petai seed extract has the same effective effect as Glimepiride to reduce blood sugar levels in alloxan-induced mice.